column_obj != None will produce a IS NOT NULL constraint:

In a column context, produces the clause a != b. If the target is None, produces a IS NOT NULL.

or use isnot() (new in 0.7.9):

Implement the IS NOT operator.

Normally, IS NOT is generated automatically when comparing to a value of None, which resolves to NULL. However, explicit usage of IS NOT may be desirable if comparing to boolean values on certain platforms.

Demo:

>>> from sqlalchemy.sql import column
>>> column('YourColumn') != None
<sqlalchemy.sql.elements.BinaryExpression object at 0x10c8d8b90>
>>> str(column('YourColumn') != None)
'"YourColumn" IS NOT NULL'
>>> column('YourColumn').isnot(None)
<sqlalchemy.sql.elements.BinaryExpression object at 0x104603850>
>>> str(column('YourColumn').isnot(None))
'"YourColumn" IS NOT NULL'