For Python 3.5+ use:

import importlib.util
spec = importlib.util.spec_from_file_location("module.name", "/path/to/file.py")
foo = importlib.util.module_from_spec(spec)
spec.loader.exec_module(foo)
foo.MyClass()

For Python 3.3 and 3.4 use:

from importlib.machinery import SourceFileLoader

foo = SourceFileLoader("module.name", "/path/to/file.py").load_module()
foo.MyClass()

(Although this has been deprecated in Python 3.4.)

For Python 2 use:

import imp

foo = imp.load_source('module.name', '/path/to/file.py')
foo.MyClass()

There are equivalent convenience functions for compiled Python files and DLLs.

See also http://bugs.python.org/issue21436.